What is a phosphate buffer and how is it used?

A phosphate buffer is a solution that maintains a stable pH level, specifically at pH 9.0 in this case. It is widely used in biological and biochemical applications, making it essential for experiments requiring a specific pH environment. By resisting changes in acidity or alkalinity, phosphate buffers are ideal for cell culture, enzyme reactions, and various laboratory procedures where pH stability is critical.

Why is a pH of 9.0 significant for phosphate buffers?

A pH of 9.0 is significant because it provides an alkaline environment suitable for various biochemical reactions. Many enzymes and proteins exhibit optimal activity at this pH, making phosphate buffers with this specific pH crucial in research and industry. For example, alkaline phosphatase, an enzyme used in molecular biology, thrives in such conditions, enhancing its functionality in experiments.

How should the phosphate buffer be stored?

Phosphate buffer should be stored in a cool, dry place, away from direct sunlight. Ideally, it should be kept in a tightly sealed, light-resistant container to prevent degradation. Proper storage is essential to maintain its effectiveness and stability, especially if you plan to use it across multiple experimental setups. For extended use, refrigerating can enhance longevity.

Can I use phosphate buffer in my laboratory experiments?

Yes, phosphate buffer is ideal for laboratory experiments, particularly those involving biochemical and biological assays. Its pH stability makes it suitable for cell cultures, DNA manipulation, and enzyme studies. When you're conducting experiments that require consistent environmental conditions, using a phosphate buffer helps ensure the reliability of your results while maximizing enzyme activity and reducing variability.

What are some common applications for phosphate buffers?

Phosphate buffers are commonly used in molecular biology, protein purification, and microbiological culture media. They are essential in applications like western blotting, enzyme assays, and electrophoresis, where maintaining a consistent pH is crucial. By providing a controlled environment, phosphate buffers enhance experimental accuracy and reproducibility, which are paramount in research settings.

Is the phosphate buffer toxic or hazardous?

The phosphate buffer at pH 9.0 is generally considered safe for use in laboratory settings. However, as with any chemical, it should be handled with care. Always utilize personal protective equipment (PPE) such as gloves and safety goggles, and ensure good ventilation. Familiarizing yourself with the material safety data sheet (MSDS) is advisable to understand any specific precautions.

What is the significance of the 0.5 M concentration?

The 0.5 M concentration indicates a moderate buffer capacity, meaning it can effectively resist changes in pH upon the addition of acids or bases. This concentration is particularly useful for experiments requiring reliable pH conditions without overpowering reactions. It allows flexibility in various applications while providing sufficient buffering to maintain a stable environment.

How can I prepare a phosphate buffer at pH 9.0?

To prepare a phosphate buffer at pH 9.0, you'll need to mix disodium hydrogen phosphate (Na2HPO4) and potassium dihydrogen phosphate (KH2PO4) in the correct proportions. By adjusting the amounts and measuring the pH, you can achieve your desired buffer solution. This method allows you to customize the buffer for specific experimental needs while ensuring it meets the pH requirements.

What is the shelf life of this phosphate buffer?

The shelf life of phosphate buffers typically ranges from several months to a year, depending on storage conditions. For optimal preservation, keep the buffer in a cool, dark place and avoid contamination. Always check for any visible signs of degradation before use. Proper storage practices can maintain its effectiveness for a longer duration, ensuring you have a reliable buffer when needed.

Where can I buy Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml?

You can purchase Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml on Ubuy in Colombia. Ubuy provides a convenient online shopping experience with a wide range of laboratory supplies, making it easy to find the specific buffer requirements for your experiments.

¿Cómo comprar Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml en línea desde Ubuy?

Es fácil comprar Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml disponible para comprar en línea en Colombia?

Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

eCon Lab Supply Ver el original

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

Our biological buffers are essential for protein research, molecular biology, and diagnostics, manufactured in an ISO certified facility for guaranteed quality.

eCon Lab Supply

Nº de artículo: 145283146

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

Nº de artículo: 145283146

COP 265859

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from Estados Unidos}

Disponibilidad : En stock

Importado de la tienda USA

QTY:

Haz tu pedido ahora y recíbelo por ahí Sábado, Octubre 10

Secured transaction

Our Top Logistics Partners

Our biological buffers are essential for protein research, molecular biology, and diagnostics, manufactured in an ISO certified facility for guaranteed quality.

Garantía U-Care:

Ninguno

Selecciona un plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Pre-Prepared Solution

This phosphate buffer is ready to use, saving time and effort in preparation, ensuring consistency in experiments and reliability for researchers requiring a stable pH of 9.0.

High Concentration

With a 0.5 M concentration, this buffer provides superior buffering capacity, effectively maintaining pH levels during biochemical reactions, making it ideal for a variety of laboratory applications.

Versatile Applications

Suitable for various biochemical and molecular biology experiments, this buffer facilitates optimal conditions for reactions such as enzyme assays and protein purification, enhancing experimental outcomes.

Detalles de producto

Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.78
Item Weight	8 ounces (226.8 grams)
Brand	eCon Lab Supply

Who Should Buy?

Biochemical Research
Ideal for scientists conducting experiments requiring stable pH conditions in biochemical assays and reactions.
Molecular Biology
Useful for researchers performing DNA/RNA extractions, where maintaining pH is critical to ensure integrity.
Cell Culture
Beneficial for cell culture applications, particularly those requiring a stable pH for optimal cell growth and function.

Acidic Environments
Not suitable for applications requiring acidic conditions, as this buffer maintains a pH of 9.0.

DESCRIPCIÓN DEL PRODUCTO

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

¿Tienes alguna consulta? Chatea con nosotros

Preguntas y respuestas de los clientes

Pregunta: ¿Cómo comprar Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml en línea desde Ubuy?

Respuesta: Es fácil comprar Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml disponible para comprar en línea en Colombia?

Respuesta: Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

eCon Lab Supply Buffers Editorial Review

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml is a reliable solution from eCon Lab Supply, offering consistent pH levels for a variety of laboratory applications. This buffer is supplied in a convenient 250 ml bottle, making it easy to use and store. Users have appreciated its stability and effectiveness in maintaining pH, essential in biochemical experiments. Additionally, it is essential to always verify compatibility with other reagents in your experiments, ensuring optimal results. With careful handling, this phosphate buffer serves well in various laboratory settings, affirming its reputation as a dependable product.

Customer Reviews & Ratings

4.0

1 valoraciones de los clientes

5 estrella

0%
4 estrella

100%
3 estrella

0%
2 estrella

0%
1 estrella

0%

Revisar este producto

Comparte tus ideas con otros clientes

ventajas

500 ml size ideal for laboratory use
Reliable pH of 9.0 for various applications
Lightweight design for easy handling
Shipped in a sturdy bottle
Trusted brand with good laboratory reputation

Contras

May not be suitable for all experiments requiring different pH levels

Product Price History

Información importante

Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

COP 265859

Haz tu pedido ahora y recíbelo por ahí Sábado, Octubre 10

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

características y beneficios

Comprehensive range of biological buffers for research and diagnostics.
Ideal for protein research, molecular biology, and characterization.
Manufactured in a USA facility certified to ISO 9001 and 13485.
Produced under stringent Good Manufacturing Practices.
Each batch undergoes rigorous quality control tests.
Popular for immunochemical staining and preserving living tissues.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

COP 265859

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Detalles de producto

Who Should Buy?

DESCRIPCIÓN DEL PRODUCTO

Preguntas y respuestas de los clientes

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Revisar este producto

ventajas

Contras

Product Price History

Información importante

COP 265859

características y beneficios

Ubuy Assurance

Fast
Shipping

Free
Return^*