What is Citrate Buffer 0.1M used for?

Citrate Buffer 0.1M is primarily used in biochemical and molecular biology applications, including enzyme assays and electrophoresis. This buffer helps maintain a stable pH environment for various biological reactions, which is crucial for accurate experimental results. For example, researchers using this buffer can carry out protein electrophoresis without pH fluctuations that could affect the migration of proteins.

Why is it important to maintain a specific pH when using Citrate Buffer?

Maintaining a specific pH, especially in the range of 4.6 to 6.2 for Citrate Buffer, is critical for enzyme activity and stability. Many enzymes require a precise pH to function optimally, and deviations can lead to denaturation or inactivation. For scientists in enzymology, using this buffer ensures that enzymatic reactions occur under ideal conditions, providing reliable data and reproducibility in experiments.

How should Citrate Buffer 0.1M be stored?

Citrate Buffer 0.1M should be stored at room temperature in a tightly sealed container to prevent contamination. Avoid exposure to direct sunlight or extreme temperatures to maintain pH stability. For use in laboratory settings, proper storage ensures that the buffer retains its effectiveness for applications such as cell culture or molecular analyses, where pH consistency is paramount.

Can I use Citrate Buffer 0.1M for cell culture?

Yes, Citrate Buffer 0.1M can be used in specific cell culture applications, although it’s generally more common in biochemical experiments. It can help maintain appropriate pH levels when culturing sensitive cell lines. Researchers often rely on such buffers when performing assays where the pH must be strictly regulated to prevent cellular stress and ensure optimal cell growth during experiments.

What are the main components of Citrate Buffer?

Citrate Buffer is primarily composed of citric acid and its corresponding salts, such as sodium citrate. These components allow for effective buffering capacity within the specified pH range. The interplay between citric acid and its conjugate base stabilizes the pH during chemical reactions, making it a reliable choice for various laboratory applications where precise pH control is essential.

Is Citrate Buffer 0.1M suitable for PCR amplification?

Citrate Buffer 0.1M is generally not the first choice for PCR amplification, as PCR typically requires a more neutral pH for optimal enzyme function. However, it can be useful in specific pre-PCR preparations or certain protocols where maintaining a slightly acidic environment is necessary. Always refer to the specific protocol details or literature for best practices when using buffers in PCR.

What techniques can benefit from using Citrate Buffer?

Techniques such as gel electrophoresis, enzyme assays, and immunoassays can greatly benefit from using Citrate Buffer. The ability to maintain a consistent pH helps ensure that reactions proceed without interference. For example, in protein gel electrophoresis, using the correct buffer allows for accurate separation based on charge and size, leading to clearer results and more reliable analysis.

Can Citrate Buffer be prepared fresh, or is it better to buy it ready-made?

Both options are valid; Citrate Buffer can be prepared fresh based on specific concentrations and pH requirements, allowing for customization per protocol needs. However, purchasing ready-made Citrate Buffer ensures consistent quality and saves time in routine laboratory work. For researchers conducting multiple experiments, ready-made options streamline processes while maintaining reliability and reproducibility in results.

Is Citrate Buffer 0.1M compatible with other buffers?

Citrate Buffer 0.1M can be compatible with other buffers; however, mixing them should be approached with caution as it may lead to unexpected pH changes. Researchers often choose to use citrate as a standalone buffer to ensure optimal performance in enzymatic reactions or biochemical assays. It’s best suited to applications that specifically require its buffering properties without complicating the reaction conditions.

Where can I buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL?

You can buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL from Ubuy. Ubuy offers a wide range of laboratory supplies, ensuring that you have access to quality products for your scientific needs.

¿Cómo comprar Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL en línea desde Ubuy?

Es fácil comprar Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL disponible para comprar en línea en Colombia?

Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Nº de artículo: 79978951

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

Nº de artículo: 79978951

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Precise pH Range

Maintains a stable pH level between 4.6 and 6.2, ensuring accurate results in various laboratory experiments and applications.

Large Volume

Supply of 500 mL is ideal for extended usage in multiple experiments, reducing the need for frequent reordering and boosting efficiency.

High Purity

Formulated with high-grade chemicals, guaranteeing minimal contaminants for reliable and reproducible results in sensitive scientific research.

Detalles de producto

Manufactured in USA under ISO 9001 and 13485 certified facility
For research use only, not for human or animal clinical/therapeutic use
Each production lot undergoes rigorous quality control tests in certified 14644 clean room conditions
Ideal for RNA isolation and antigen detection in fixation medium
Popular choice for immunofluorescent staining applications
Comprehensive range of biological buffers for research, diagnostics, and drug development

Item Form	Liquid
Item Weight	12 ounces (340.2 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.129
Brand	eCon-Lab-Supply
Item Volume	500 ml

Who Should Buy?

Laboratory Scientists
Ideal for scientists conducting experiments requiring stable pH levels in biochemical and molecular biology applications.
Educational Institutions
Useful for schools and universities teaching laboratory techniques in chemistry and biology involving pH buffers.
Pharmaceutical Research
Essential for researchers working on drug formulations where precise pH maintenance is crucial for stability and efficacy.

General Home Use
Not suitable for household applications, as it is a specialized laboratory chemical not typically needed at home.

DESCRIPCIÓN DEL PRODUCTO

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

About This Item

Looking for a reliable citrate buffer for your biochemical applications? Look no further than our 0.1M Citrate Buffer, offering a pH range of 4.6-6.2. With a generous volume of 500 mL, this high-quality citrate buffer is perfect for your lab needs. Whether you are conducting research or carrying out experiments, our citrate buffer ensures consistent and accurate results.

Buy online now and experience the best in citrate buffering solutions.

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Pregunta: ¿Cómo comprar Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL en línea desde Ubuy?

Respuesta: Es fácil comprar Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL disponible para comprar en línea en Colombia?

Respuesta: Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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ventajas

Accurate pH range (4.6-6.2)
Convenient 500 mL size
Excellent for biochemical applications
Stable formulation and long shelf life
Compatible with various assays

Contras

Limited use outside of pH range

Product Price History

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Comprehensive range of biological buffers
Suitable for research, diagnostics, drug development, and manufacturing
Manufactured in an ISO 9001 and 13485 certified facility
Each production lot manufactured under stringent quality control tests
Citrate buffers prevent base hydrolysis and break cross-links between antigens
Popular choice for immunofluorescent staining applications

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Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

COP 202540

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What Stands Out

Detalles de producto

Who Should Buy?

DESCRIPCIÓN DEL PRODUCTO

About This Item

Preguntas y respuestas de los clientes

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Revisar este producto

ventajas

Contras

Product Price History

Información importante

COP 202540

características y beneficios

Ubuy Assurance

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