What is 10X Phosphate Buffered Saline (PBS)?

10X Phosphate Buffered Saline (PBS) is a concentrated buffer solution that maintains a stable pH environment, particularly at 7.4. It is widely used in biological research, especially in cell culture and molecular biology applications. The high concentration enables researchers to dilute it to a working concentration appropriate for their experiments. This buffered saline helps maintain osmotic balance and provides essential ions needed for cellular functions.

What are the applications of 10X PBS?

10X PBS is versatile and used in various applications including cell culture, protein purification, and biochemical assays. Because it maintains the pH and ionic strength, it is ideal for diluting cells, preparing samples for western blotting, or as a wash buffer in immunoassays. For instance, a scientist might use it to resuspend cells before conducting experiments, ensuring they remain viable and functional.

How should 10X PBS be prepared for use?

To use 10X PBS, it should be diluted to a 1X working solution by mixing it with sterile distilled water in a 1:9 ratio. This means adding one part of the 10X PBS to nine parts of water. This dilution process is crucial as it creates a buffer that mimics physiological conditions, which is essential for biological studies and experiments.

Typically, 10X PBS is not inherently sterile unless specifically labeled as such. If you're using it for sensitive biological applications, it’s advisable to filter-sterilize the diluted 1X solution or purchase pre-sterilized PBS. This precaution helps prevent contamination in cell cultures or sensitive assays, ensuring that experimental results are reliable and reproducible.

Can 10X PBS be stored after dilution?

It is recommended to use the 1X PBS solution freshly prepared; however, if necessary, it can be stored at 4°C for short periods. Longer storage may lead to changes in pH or contamination. To maintain the integrity of your work, consider preparing smaller batches that can be used within a few days, thus reducing the risk of degradation or bacterial growth.

What components make up 10X PBS?

10X PBS typically consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. This combination provides the necessary ionic strength and buffering capacity to stabilize the pH during biological experiments. The particular balance of these components is essential for maintaining osmotic balance, making it compatible with living cells and tissues.

How does the pH of 7.4 benefit biological experiments?

A pH of 7.4 is physiological, mimicking the natural environment of human body fluids. This property is vital for experiments involving cell cultures and enzymatic reactions as it supports optimal protein function and cellular behavior. For example, maintaining this pH is crucial when working with mammalian cells to ensure their growth and responsiveness during research.

Is there a difference between 1X PBS and 10X PBS?

Yes, the primary difference is their concentration. 10X PBS is a concentrated stock solution that needs to be diluted to achieve a 1X concentration ideal for biological work. The 10X stock allows for greater convenience in storage and long-term use, providing flexibility in preparing various volumes of dilutions suited for specific experimental needs.

Where can I buy 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL in Colombia?

You can purchase 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL from Ubuy. They offer a wide selection of laboratory products, ensuring you find the right item for your research needs. Ubuy provides a platform where you can easily browse and select necessary scientific supplies.

What safety precautions should be taken when using 10X PBS?

Standard laboratory safety precautions should be followed. Always wear protective gear, including gloves and goggles, when handling reagents. Ensure that you work in a sterile environment to minimize the risk of contamination in biological experiments. Additionally, proper labeling and storage protocols should be followed to ensure the integrity of the PBS and the safety of the user.

¿Cómo comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL en línea desde Ubuy?

Es fácil comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL disponible para comprar en línea en Colombia?

Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

10X Phosphate Buffered Saline is a balanced, isotonic salt solution essential for a variety of cell culture applications.

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10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

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Nº de artículo: 127455181

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10X Phosphate Buffered Saline is a balanced, isotonic salt solution essential for a variety of cell culture applications.

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High Concentration

The 10X formulation offers a robust concentration, making it ideal for dilutions required in various biological and laboratory applications, ensuring accurate results and optimal performance for researchers.

pH Stability

Maintaining a pH of 7.4 ensures a biological compatibility that is crucial for cell culture and biochemical assays, providing researchers with a reliable buffer that mimics physiological conditions.

Bulk Volume

The 500mL packaging caters to high-demand laboratory settings, reducing the frequency of reordering and ensuring that labs have sufficient quantities for extensive studies or experiments.

Detalles de producto

Container Type	Bottle
Item Form	Liquid
Item Weight	16 ounces (453.6 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.03
Brand	eCon Lab Supply
Item Volume	500 ml
Package Information	Bottle

Who Should Buy?

Cell Biology Research
Ideal for cell cultures, maintaining optimal pH and osmotic balance for various biological experiments.
Molecular Biology Use
Recommended for applications like protein purification and cloning that require stable buffer systems.
Diagnostic Applications
Useful in laboratories for preparing samples and diluting reagents in diagnostics and assays.

Non-Laboratory Use
Not suitable for home or casual use, as it is intended for professional laboratory environments.

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10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

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Pregunta: ¿Cómo comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL en línea desde Ubuy?

Respuesta: Es fácil comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL disponible para comprar en línea en Colombia?

Respuesta: Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

eCon Lab Supply Buffers Editorial Review

10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL is a highly versatile liquid solution in a 500 mL bottle from eCon Lab Supply, ideal for various laboratory applications. This phosphate buffered saline is essential for cell culture, providing a stable pH environment crucial for biological activities. The liquid form ensures easy handling and precise measurements, making it suitable for experimental procedures requiring reliable phosphate buffering. Users have noted its effectiveness in maintaining cell viability during experimentation, highlighting its importance in research and biological assays.

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ventajas

Convenient liquid form for easy use
Stable pH at 7.4 for reliable results
Packaged in a 500 mL bottle for ample supply
Essential for cell culture and biological assays
Trusted brand known for quality laboratory supplies

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Might require specific storage conditions for optimal longevity

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Manufactured in a certified US facility adhering to rigorous quality control.
Contains essential components for biological research: NaCl, NaH2PO4, KCl, KH2PO4.
0.22 micron filter-sterilized for optimal purity.
Ideal for washing, transporting, and diluting cells.
Available in convenient 500 mL and 1L sizes.
Strictly for research use only, ensuring high-quality outcomes.

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10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

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eCon Lab Supply Buffers Editorial Review

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