What is a Phosphate Buffer, pH 6.5 [0.5 M] used for?

A Phosphate Buffer, pH 6.5 [0.5 M] is primarily used to maintain a stable pH in various biological and chemical experiments. This buffer is essential in contexts like enzymatic reactions, where pH can significantly affect enzyme activity and stability. In laboratory settings, it acts as a stabilizing solution for pH-sensitive processes, ensuring accurate results in procedures like DNA extraction or protein assays.

How do you prepare a Phosphate Buffer, pH 6.5?

Preparing a Phosphate Buffer at pH 6.5 involves mixing specific volumes of dihydrogen phosphate and hydrogen phosphate salts with distilled water. Typically, you'd calculate the required amounts based on the buffer's total volume. It’s important to adjust the pH accurately using a pH meter, which may involve adding small quantities of acid or base to reach the desired pH level. This process ensures that the buffer is effective for applications in various experiments.

Can the Phosphate Buffer, pH 6.5 be stored for future use?

Yes, the Phosphate Buffer, pH 6.5 can be stored for future use, typically in a sterile, closed container to prevent contamination. For optimal results, store it at room temperature or refrigerated, and ensure to check the pH before using it in experiments. This makes it convenient for labs that frequently require pH stabilization without the need to prepare fresh solutions each time.

Is the Phosphate Buffer suitable for cell culture applications?

Absolutely, the Phosphate Buffer, pH 6.5 is suitable for cell culture applications. It mimics physiological conditions, providing a stable environment that helps maintain cellular activities during experimentation. This buffer is essential when working with mammalian cells or bacteria, as fluctuations in pH can adversely affect cell behavior and metabolic functions. Thus, it’s widely used by researchers in cell biology studies.

What makes Phosphate Buffer, pH 6.5, preferable over other buffers?

The Phosphate Buffer at pH 6.5 is often preferred due to its effective buffering capacity close to physiological pH. Phosphate buffers are less likely to interfere with biological reactions compared to some organic buffers. Additionally, they are cost-effective, easy to prepare, and compatible with a wide range of biological systems. These characteristics make it an ideal choice for many laboratory applications.

What are the safety considerations for using Phosphate Buffers?

When using Phosphate Buffers, pH 6.5, typical safety precautions include wearing gloves and goggles to avoid skin and eye irritation. The chemicals involved are generally safe, but it's vital to ensure proper ventilation in the laboratory. Always refer to the Material Safety Data Sheet (MSDS) for specific handling and disposal guidelines. This ensures a safe working environment while working with scientific reagents.

Can the Phosphate Buffer be used in biochemical assays?

Yes, Phosphate Buffer, pH 6.5 is frequently used in biochemical assays to ensure optimal pH conditions for enzyme activity. Many assays, including those assessing enzymatic reactions, depend on maintaining a stable pH, and this buffer fulfills that requirement effectively. It’s an excellent choice for assays involving nucleic acids or proteins, where pH fluctuations can lead to inaccurate results.

What volume of Phosphate Buffer should I use in my experiments?

The volume of Phosphate Buffer to use in experiments varies based on specific experimental requirements. Commonly, researchers use anywhere from 5 to 50 ml, depending on the scale of the experiment and the number of samples being processed. For micro-scale experiments, smaller volumes may suffice, while larger studies may require higher volumes to maintain pH stability across samples.

Where can I buy Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml in Colombia?

You can buy Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml on Ubuy in Colombia. Ubuy offers a wide range of laboratory chemicals and supplies, making it a reliable option for those needing specific reagents for research or educational purposes.

Is there a difference between this buffer and other pH buffers?

Yes, there is a significant difference between Phosphate Buffer, pH 6.5 and other types of pH buffers. Each buffer has a specific pH range in which it is effective. Phosphate buffers are particularly good for biological studies due to their competence in maintaining pH stability within the physiological range. In contrast, other buffers might not perform as well in biological contexts. Choosing the right buffer ensures reliable experimental conditions.

¿Cómo comprar Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml en línea desde Ubuy?

Es fácil comprar Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml disponible para comprar en línea en Colombia?

Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our biological buffers are crucial for protein research and molecular biology.

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Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

Nº de artículo: 125268547

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Precise pH Control

Maintains a stable pH of 6.5, providing accuracy in experiments and applications requiring strict pH consistency.

High Concentration

At 0.5 M, this buffer offers effective resistance to pH changes, making it suitable for sensitive biochemical reactions.

Convenient Size

The 250 ml volume is ideal for various laboratory applications, minimizing waste while maximizing usability in research and educational settings.

Detalles de producto

Container Type	Bottle
Item Form	Liquid
Item Weight	8 ounces (226.8 grams)
Volume	250 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.75
Brand	eCon Lab Supply
Item Volume	250 ml
Package Information	Bottle

Who Should Buy?

Biological Research
Ideal for experiments requiring stable pH, suitable for cellular and molecular biology applications.
Laboratory Experiments
Perfect for use in various chemistry labs that demand precise pH control during experiments.
Protein Studies
Beneficial in maintaining optimal conditions for protein purification and enzymatic reactions at pH 6.5.

High pH Applications
Not suitable for experiments requiring a pH above 7, as it is specifically formulated at pH 6.5.

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Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

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Pregunta: ¿Cómo comprar Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml en línea desde Ubuy?

Respuesta: Es fácil comprar Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml disponible para comprar en línea en Colombia?

Respuesta: Sí, en Ubuy Colombia, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Comprehensive range of biological buffers.
Ideal for research, diagnostics, drug development, and manufacturing.
Manufactured in an ISO 9001 and 13485 certified facility in the USA.
Stringently produced under Good Manufacturing Practices.
Quality control under certified 14644 clean room conditions.
Essential for immunochemical staining and preserving living tissues.

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Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

COP 265859

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eCon Lab Supply Buffers Editorial Review

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COP 265859

características y beneficios

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